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How does LC column packing affect column energy?

How does LC column packing affect column energy?

The performance of LC columns depends primarily on the nature and type of packing. The size, shape, bonded phase, carbon content, etc. of the packing can all affect the performance of the liquid chromatography column, which is very helpful for our use and selection of chromatography columns.

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Causes and Solutions of Gas Chromatography Peak Tailing(Ⅱ)

Causes and Solutions of Gas Chromatography Peak Tailing(Ⅱ)

When analyzing compounds with gas chromatography (GC) and gas chromatography-mass spectrometry, sometimes you will encounter the problem of chromatographic peak tailing, which is mainly analyzed from the following aspects:

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Causes and Solutions of Gas Chromatography Peak Tailing(Ⅰ)

Causes and Solutions of Gas Chromatography Peak Tailing(Ⅰ)

When analyzing compounds with gas chromatography (GC) and gas chromatography-mass spectrometry, sometimes you will encounter the problem of chromatographic peak tailing, which is mainly analyzed from the following aspects:

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What Size Syringe Filter for HPLC? And How to Use It?

What Size Syringe Filter for HPLC? And How to Use It?

Syringe filters provide high flow rates and loading capacities, making them ideal for sample preparation for HPLC and ion chromatography. Their use extends column lifetime significantly, while their variety of membrane types and pore sizes makes them a versatile choice. Get the most out of your chromatography data with the right filter. When choosing a syringe filter, three main factors dictate your syringe filter selection: sample volume, potential particulate size, and filter membrane compatibility.

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How do you select diluent in HPLC?

How do you select diluent in HPLC?

In this article, we will explore the importance of selecting the right diluent in HPLC and provide practical guidelines to help you make informed choices for your specific analytical needs.

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One of the “Three Key Points” for HPLC Instrument Operation: Filtration(1)

One of the “Three Key Points” for HPLC Instrument Operation: Filtration(1)

Any particulate matter entering the HPLC system will be blocked by the sieve plate at the inlet end of the column. The final result will be blockage of the column, which is characterized by increased system pressure and deformation of the chromatographic peaks. Therefore, various preventive measures should be taken, including operating procedures and various filtration designs of commercial instruments themselves, in an effort to prevent or reduce particulate matter from entering the HPLC system, thereby extending the service life of the instrument and chromatographic column, and improving the reliability of data.

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One of the “Three Key Points” for HPLC Instrument Operation: Mobile Phase Degassing

One of the “Three Key Points” for HPLC Instrument Operation: Mobile Phase Degassing

Degassing the mobile phase is a very effective measure to avoid problems with the HPLC system and to obtain ideal data smoothly. It is undesirable to have air bubbles in the system. HPLC pumps must generate a lot of force when transporting liquids.

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When using micro inserts in HPLC analysis, are there any special instructions or considerations?

When using micro inserts in HPLC analysis, are there any special instructions or considerations?

Micro inserts are valuable accessories in HPLC analysis, designed to enhance the accuracy and efficiency of chromatographic experiments. These tiny devices play a crucial role in sample containment and injection precision, ensuring optimal performance and reliable results.

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Analysis and treatment methods of causes of chromatographic peak tailing(Ⅱ)

Analysis and treatment methods of causes of chromatographic peak tailing(Ⅱ)

Last time we explained the causes of liquid chromatography column tailing. Today we will explain the causes of gas chromatography peak tailing.
Generally speaking, when dealing with gas chromatography peak tailing problems, it can be summed up in one sentence: XXX sample is tailing on XXX chromatographic column. What is the reason?

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Static Headspace-gas chromatography Sample Introduction Method

Static Headspace-gas chromatography Sample Introduction Method

There are different sample introduction methods for gas chromatograph static headspace sampling, and you can choose the appropriate sampling method according to your own needs to reduce unnecessary troubles. Static headspace intrductions are available as syringe injections, equilibrium pressurized injections and dosing ring pressurized injections.

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